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We know the terms Ex vivo and In vitro are very close but they are not same.. non of the articles gives satisfactory information in differentiating these terms.and with
Although our approach to the clinical management of osteoporosis OP and degenerative joint diseases DJD -major causes of disability and morbidity in the elderly-has greatly advanced in the past decades, curative treatments that could bring ultimate solutions have yet to be found or developed. Effective and timely development of candidate drugs is a critical function of the availability of sensitive and accurate methodological arsenal enabling the recognition and quantification of pharmacodynamic effects. The established concept that both OP and DJD arise from an imbalance in processes of tissue formation and degradation draws attention to need of establishing in vitro, ex vivo, and in vivo experimental settings, which allow obtaining insights into the mechanisms driving increased bone and cartilage degradation at cellular, organ, and organism levels. When addressing changes in bone or cartilage turnover at the organ or organism level, monitoring tools adequately reflecting the outcome of tissue homeostasis become particularly critical. In this context, bioassays targeting the quantification of various degradation and formation products of bone and cartilage matrix elements represent a useful approach.
What is the difference between Ex vivo, In vivo and In vitro?
Ex vivo Latin : "out of the living" means that which takes place outside an organism. In science, ex vivo refers to experimentation or measurements done in or on tissue from an organism in an external environment with minimal alteration of natural conditions.
Angiogenesis is a biological process with a central role in retinal diseases. The choice of the ideal method to study angiogenesis, particularly in the retina, remains a problem. Angiogenesis can be assessed through in vitro and in vivo studies. In spite of inherent limitations, in vitro studies are faster, easier to perform and quantify, and typically less expensive and allow the study of isolated angiogenesis steps. We performed a systematic review of PubMed searching for original articles that applied in vitro or ex vivo angiogenic retinal assays until May , presenting the available assays and discussing their applicability, advantages, and disadvantages. Most of the studies evaluated migration, proliferation, and tube formation of endothelial cells in response to inhibitory or stimulatory compounds. Other aspects of angiogenesis were studied by assessing cell permeability, adhesion, or apoptosis, as well as by implementing organotypic models of the retina.
In vitro Latin: in glass studies in experimental biology are those that are conducted using components of an organism that have been isolated from their usual biological surroundings in order to permit a more detailed or more convenient analysis than can be done with whole organisms. Colloquially, these experiments are commonly called "test tube experiments". In contrast, in vivo work is that which is conducted with living organisms in their normal, intact state, while ex vivo studies are conducted on functional organs that have been removed from the intact organism. Common examples of in vitro experiments include work that uses a cells derived from multicellular organisms cell culture or tissue culture , b subcellular components e. In vivo Latin for "within the living" is experimentation using a whole, living organism as opposed to a partial or dead organism, or an in vitro "within the glass", i. Animal testing and clinical trials are two forms of in vivoresearch. In vivo testing is often employed over in vitro because it is better suited for observing the overall effects of an experiment on a living subject.
In vitro research is foundational to all biomedical research. The advancements made in studying cells, bacteria, and viruses are often the first strides into understanding the in vivo condition. We offer a laboratory solution for providing a stable physiologically relevant environment to cells as well as a solution to count colonies in colony forming assays. Ex vivo research is key to understanding specific organ and tissue function removed from other bodily stimuli. We currently offer many isolated heart, tissue, and vessel options. For studies requiring a physiologically accurate atmosphere the HypoxyLab offers full control of O 2 , CO 2 , temperature and humidity. This ergonomically designed benchtop system is the only one of its kind to take measurements using the partial pressure of oxygen, making it the most reliable system for measuring oxygen on the market.